CELL CULTURE
Scientific Supervisor: Gemma Fabriàs
The Cell Culture Service is a CID scientific-tecnic support unit that offers to IQAC and IDAEA research groups and external laboratories the equipment and appropiate facilities to carry out the culture and maintenance of animal cell lines as well as specialized staff for performing bioassays in several aspects of the biomedical and toxicological sciences
Gemma Fabrias
Scientific Supervisor
gemma.fabrias@iqac.csic.es
Ignacio Perez
Technical Supervisor
ignacio.perez@iqac.csic.es
The Service provide biosafety level 2 facilities authorized by appropriate organizations, suitable for work involving agents of moderate potential hazard to personnel and the environment.
Cell culture laboratory access is allowed only to authorized personnel, who must know general and specific service standards by making an specific course that involves:
– Security and waste treatment meassures.
– Organization standars.
– Training in use of laboratory equipment.
SERVICES
- Cell propagation and stock maintenance.
- Cryopreservation of viable cells.
- Performance testing of fetal bovine serum
- Mycoplasma screening in cell cultures by PCR
- Personnel training on basic techniques of cell cultures.
- Maintenance of facilities and equipment.
- Performance of bioassays upon request.
RATES
EQUIPMENT
- Vertical laminar flow cabinets.
- Class II Biosafety cabinets.
- CO2 incubators.
- Refrigerators and freezers.
- Autoclave.
- Inverted microscope.
- Fluorescence microscope equipped with a digital camera.
- High resolution fluorescence microscope.
- Countess automatic cell counter.
- Guava EasyCyte flow cytometer.
- Water baths.
- Tabletop refrigerated centrifuge.
- Vortex.
- Liquid nitrogen tanks for cell storage.
