CELL CULTURE
Scientific Supervisor: Gemma Fabriàs
The Cell Culture Service is a CID scientific-tecnic support unit that offers to IQAC and IDAEA research groups and external laboratories the equipment and appropiate facilities to carry out the culture and maintenance of animal cell lines as well as specialized staff for performing bioassays in several aspects of the biomedical and toxicological sciences
Gemma Fabrias
Scientific Supervisor
gemma.fabrias@iqac.csic.es
Ignacio Perez
Technical Supervisor
ignacio.perez@iqac.csic.es
The Service provide biosafety level 2 facilities authorized by appropriate organizations, suitable for work involving agents of moderate potential hazard to personnel and the environment.
Cell culture laboratory access is allowed only to authorized personnel, who must know general and specific service standards by making an specific course that involves:
– Security and waste treatment meassures.
– Organization standars.
– Training in use of laboratory equipment.
SERVICES
- Cell propagation and stock maintenance.
- Cryopreservation of viable cells.
- Performance testing of fetal bovine serum
- Mycoplasma screening in cell cultures by PCR
- Personnel training on basic techniques of cell cultures.
- Maintenance of facilities and equipment.
- Performance of bioassays upon request.
RATES
EQUIPMENT
- Vertical laminar flow cabinets.
- Class II Biosafety cabinets.
- CO2 incubators.
- Refrigerators and freezers.
- Autoclave.
- Inverted microscope.
- Fluorescence microscope equipped with a digital camera.
- High resolution fluorescence microscope.
- Countess automatic cell counter.
- Guava EasyCyte flow cytometer.
- Water baths.
- Tabletop refrigerated centrifuge.
- Vortex.
- Liquid nitrogen tanks for cell storage.
- Cytotoxicity assessment and suspected screening of PLASTIC ADDITIVES in bioplastics of single-use household items-
Katerina Savva, Xavier Borrell, Teresa Moreno, Ignacio Pérez-Pomeda, Carlos Barata, Marta Llorca, Marinella Farré
Chemosphere (2023),
- Peptide Amphiphilic-Based Supramolecular Structures with Anti-HIV-1 Activity-
Maria J. Gómara, Ramon Pons, Carolina Herrera, Paul Ziprin, and Isabel Haro
Bioconjugate Chem. 32 (9), pp. 1999-2013. 2021
- Click and count: Specific detection of acid ceramidase activity in live cells-
Casasampere, M., Izquierdo, E., Casas, J., Abad, J.L., Liu, X., Xu, R., Mao, C., Chang, Y.-T., Delgado, A., Fabrias, G.
Chemical Science, 11 (48), pp. 13044-13051. 2020
- Lipid Vesicles Loaded with an HIV-1 Fusion Inhibitor Peptide as a Potential Microbicide-
Elena Sánchez-López, Anna Paús, Ignacio Pérez-Pomeda, Ana Calpena, Isabel Haro and María José Gómara
Pharmaceutics 2020, 12(6), 502
- Design, Characterization, and Biopharmaceutical Behavior of nanoparticles Loaded with an HIV-1 Fusion Inhibitor Peptide-
Ariza-Sáenz, M., Espina, M., Calpena, A., Gómara, M.J., Pérez-Pomeda, I., Haro, I., García, M.L.
Mol. Pharmaceutics 2018, 15, 5005−5018
- Cytotoxic effects of comonly used nanomaterials and microplastics on cerebral and epitelial human cells-
Gabriella F. Schirinzi, Ignacio Pérez-Pomeda, Josep Sanchís, Cesare Rossini, Marinella Farré, and Damià Barceló
Environmental Research, Volume 159, November 2017, Pages 579-587
- Lipid raft-like liposomes used for targeted delivery of a chimeric entry-inhibitor peptide with anti-HIV-1 activity-
María José Gómara, Ignacio Pérez-Pomeda, José María Gatell, Victor Sánchez-Merino, Eloisa Yuste, Isabel Haro
Nanomedicine: Nanotechnology, Biology and Medicine 13(2), 601-609 (2017)
