{"id":12809,"date":"2026-10-27T07:36:27","date_gmt":"2026-10-27T06:36:27","guid":{"rendered":"https:\/\/www.iqac.csic.es\/proyecto\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/"},"modified":"2026-10-29T08:33:34","modified_gmt":"2026-10-29T07:33:34","slug":"one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy","status":"publish","type":"proyecto","link":"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/","title":{"rendered":"ONE-POT assay for anti-cancer nanomedicine screening: decoding barcoded nanoparticles using DNA-PAINT microscopy"},"content":{"rendered":"

Nanomedicine emerged 20 years ago promising selective drug delivery, but few nanomedicines have reached patients. Their screening is usually done in 2D cell cultures, which are far from 3D reality. Organ-on-a-chip (OOAC) technology offers models closer to the disease, but it is complex and hard to parallelize, so it is not high-throughput. DNA-PAINT microscopy, thanks to its programmability and high multiplexing capacity, could solve this problem: short oligonucleotide sequences make it possible to barcode hundreds or thousands of nanoparticles (NPs) and analyze them simultaneously in a one-pot assay that reports which NP it is and where it is located. This project combines super-resolution microscopy and OOAC to screen anti-cancer nanomedicines in a single step. NP libraries will be prepared by varying size, geometry, and ligands, and evaluated in a breast-cancer-on-a-chip model including a tumor spheroid, stromal cells, and tumor ECM. This will provide multiparametric information on distribution, extravasation, selectivity, and efficacy, supporting a more rational design of nanomedicines.<\/p>\n

Ayuda MCIN\/AEI\/10.13039\/501100011033 Uni\u00f3n Europea \u00abNext Generation EU\u00bb\/PRTR <\/em>financiada por:<\/p>\n

\"\"<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

ONE-POT project combines DNA-PAINT super-resolution microscopy, which makes it possible to barcode hundreds or thousands of nanoparticles with DNA and analyze them simultaneously in a single assay, with organ-on-a-chip technology to screen anti-cancer nanomedicines. Using a realistic breast-cancer-on-a-chip model (with tumor spheroid, stromal cells, and ECM), multiparametric information will be obtained on the distribution, extravasation, selectivity, and efficacy of the nanoparticles, enabling a more rational design of nanomedicines.<\/p>\n","protected":false},"featured_media":12810,"parent":0,"template":"","meta":{"_acf_changed":false},"tags":[182,194],"enfermedad":[171],"framework":[155],"linea-de-investigacion":[172,163],"class_list":["post-12809","proyecto","type-proyecto","status-publish","has-post-thumbnail","hentry","tag-nanobiotechnology","tag-nanomedicine","enfermedad-cancer","framework-aei-agencia-estatal-de-investigacion","linea-de-investigacion-treat","linea-de-investigacion-understand","entry"],"acf":[],"yoast_head":"\nONE-POT assay for anti-cancer nanomedicine screening: decoding barcoded nanoparticles using DNA-PAINT microscopy | IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"ONE-POT assay for anti-cancer nanomedicine screening: decoding barcoded nanoparticles using DNA-PAINT microscopy | IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC\" \/>\n<meta property=\"og:description\" content=\"ONE-POT project combines DNA-PAINT super-resolution microscopy, which makes it possible to barcode hundreds or thousands of nanoparticles with DNA and analyze them simultaneously in a single assay, with organ-on-a-chip technology to screen anti-cancer nanomedicines. Using a realistic breast-cancer-on-a-chip model (with tumor spheroid, stromal cells, and ECM), multiparametric information will be obtained on the distribution, extravasation, selectivity, and efficacy of the nanoparticles, enabling a more rational design of nanomedicines.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/\" \/>\n<meta property=\"og:site_name\" content=\"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/iqac.csic.es\/\" \/>\n<meta property=\"article:modified_time\" content=\"2026-10-29T07:33:34+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www.iqac.csic.es\/wp-content\/uploads\/2026\/10\/colin00b-mitosis-3876669-scaled.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"2560\" \/>\n\t<meta property=\"og:image:height\" content=\"1440\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:site\" content=\"@IQAC_CSIC\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/en\\\/project\\\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\\\/\",\"url\":\"https:\\\/\\\/www.iqac.csic.es\\\/en\\\/project\\\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\\\/\",\"name\":\"ONE-POT assay for anti-cancer nanomedicine screening: decoding barcoded nanoparticles using DNA-PAINT microscopy | IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/en\\\/project\\\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/en\\\/project\\\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www.iqac.csic.es\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/colin00b-mitosis-3876669-scaled.jpg\",\"datePublished\":\"2026-10-27T06:36:27+00:00\",\"dateModified\":\"2026-10-29T07:33:34+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/en\\\/project\\\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.iqac.csic.es\\\/en\\\/project\\\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/en\\\/project\\\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\\\/#primaryimage\",\"url\":\"https:\\\/\\\/www.iqac.csic.es\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/colin00b-mitosis-3876669-scaled.jpg\",\"contentUrl\":\"https:\\\/\\\/www.iqac.csic.es\\\/wp-content\\\/uploads\\\/2026\\\/10\\\/colin00b-mitosis-3876669-scaled.jpg\",\"width\":2560,\"height\":1440},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/en\\\/project\\\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Portada\",\"item\":\"https:\\\/\\\/www.iqac.csic.es\\\/en\\\/home\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"ONE-POT assay for anti-cancer nanomedicine screening: decoding barcoded nanoparticles using DNA-PAINT microscopy\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/#website\",\"url\":\"https:\\\/\\\/www.iqac.csic.es\\\/\",\"name\":\"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC\",\"description\":\"Chemistry for a better life\",\"publisher\":{\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/#organization\"},\"alternateName\":\"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www.iqac.csic.es\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/#organization\",\"name\":\"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC\",\"alternateName\":\"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC\",\"url\":\"https:\\\/\\\/www.iqac.csic.es\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/www.iqac.csic.es\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/cropped-favicon-1.png\",\"contentUrl\":\"https:\\\/\\\/www.iqac.csic.es\\\/wp-content\\\/uploads\\\/2025\\\/11\\\/cropped-favicon-1.png\",\"width\":512,\"height\":512,\"caption\":\"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC\"},\"image\":{\"@id\":\"https:\\\/\\\/www.iqac.csic.es\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.facebook.com\\\/iqac.csic.es\\\/\",\"https:\\\/\\\/x.com\\\/IQAC_CSIC\",\"https:\\\/\\\/www.instagram.com\\\/iqac_csic\",\"https:\\\/\\\/www.youtube.com\\\/channel\\\/UC1JXTOe3-s8UnYu_A6-SI3A\",\"https:\\\/\\\/www.linkedin.com\\\/company\\\/iqac-csic\\\/\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"ONE-POT assay for anti-cancer nanomedicine screening: decoding barcoded nanoparticles using DNA-PAINT microscopy | IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/","og_locale":"en_US","og_type":"article","og_title":"ONE-POT assay for anti-cancer nanomedicine screening: decoding barcoded nanoparticles using DNA-PAINT microscopy | IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC","og_description":"ONE-POT project combines DNA-PAINT super-resolution microscopy, which makes it possible to barcode hundreds or thousands of nanoparticles with DNA and analyze them simultaneously in a single assay, with organ-on-a-chip technology to screen anti-cancer nanomedicines. Using a realistic breast-cancer-on-a-chip model (with tumor spheroid, stromal cells, and ECM), multiparametric information will be obtained on the distribution, extravasation, selectivity, and efficacy of the nanoparticles, enabling a more rational design of nanomedicines.","og_url":"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/","og_site_name":"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC","article_publisher":"https:\/\/www.facebook.com\/iqac.csic.es\/","article_modified_time":"2026-10-29T07:33:34+00:00","og_image":[{"width":2560,"height":1440,"url":"https:\/\/www.iqac.csic.es\/wp-content\/uploads\/2026\/10\/colin00b-mitosis-3876669-scaled.jpg","type":"image\/jpeg"}],"twitter_card":"summary_large_image","twitter_site":"@IQAC_CSIC","twitter_misc":{"Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/","url":"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/","name":"ONE-POT assay for anti-cancer nanomedicine screening: decoding barcoded nanoparticles using DNA-PAINT microscopy | IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC","isPartOf":{"@id":"https:\/\/www.iqac.csic.es\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/#primaryimage"},"image":{"@id":"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/#primaryimage"},"thumbnailUrl":"https:\/\/www.iqac.csic.es\/wp-content\/uploads\/2026\/10\/colin00b-mitosis-3876669-scaled.jpg","datePublished":"2026-10-27T06:36:27+00:00","dateModified":"2026-10-29T07:33:34+00:00","breadcrumb":{"@id":"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/#primaryimage","url":"https:\/\/www.iqac.csic.es\/wp-content\/uploads\/2026\/10\/colin00b-mitosis-3876669-scaled.jpg","contentUrl":"https:\/\/www.iqac.csic.es\/wp-content\/uploads\/2026\/10\/colin00b-mitosis-3876669-scaled.jpg","width":2560,"height":1440},{"@type":"BreadcrumbList","@id":"https:\/\/www.iqac.csic.es\/en\/project\/one-pot-assay-for-anti-cancer-nanomedicine-screening-decoding-barcoded-nanoparticles-using-dna-paint-microscopy\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Portada","item":"https:\/\/www.iqac.csic.es\/en\/home\/"},{"@type":"ListItem","position":2,"name":"ONE-POT assay for anti-cancer nanomedicine screening: decoding barcoded nanoparticles using DNA-PAINT microscopy"}]},{"@type":"WebSite","@id":"https:\/\/www.iqac.csic.es\/#website","url":"https:\/\/www.iqac.csic.es\/","name":"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC","description":"Chemistry for a better life","publisher":{"@id":"https:\/\/www.iqac.csic.es\/#organization"},"alternateName":"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.iqac.csic.es\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/www.iqac.csic.es\/#organization","name":"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC","alternateName":"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC","url":"https:\/\/www.iqac.csic.es\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.iqac.csic.es\/#\/schema\/logo\/image\/","url":"https:\/\/www.iqac.csic.es\/wp-content\/uploads\/2025\/11\/cropped-favicon-1.png","contentUrl":"https:\/\/www.iqac.csic.es\/wp-content\/uploads\/2025\/11\/cropped-favicon-1.png","width":512,"height":512,"caption":"IQAC - Institut de Qu\u00edmica Avan\u00e7ada de Catalunya - CSIC"},"image":{"@id":"https:\/\/www.iqac.csic.es\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/iqac.csic.es\/","https:\/\/x.com\/IQAC_CSIC","https:\/\/www.instagram.com\/iqac_csic","https:\/\/www.youtube.com\/channel\/UC1JXTOe3-s8UnYu_A6-SI3A","https:\/\/www.linkedin.com\/company\/iqac-csic\/"]}]}},"_links":{"self":[{"href":"https:\/\/www.iqac.csic.es\/en\/wp-json\/wp\/v2\/proyecto\/12809","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.iqac.csic.es\/en\/wp-json\/wp\/v2\/proyecto"}],"about":[{"href":"https:\/\/www.iqac.csic.es\/en\/wp-json\/wp\/v2\/types\/proyecto"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.iqac.csic.es\/en\/wp-json\/wp\/v2\/media\/12810"}],"wp:attachment":[{"href":"https:\/\/www.iqac.csic.es\/en\/wp-json\/wp\/v2\/media?parent=12809"}],"wp:term":[{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.iqac.csic.es\/en\/wp-json\/wp\/v2\/tags?post=12809"},{"taxonomy":"enfermedad","embeddable":true,"href":"https:\/\/www.iqac.csic.es\/en\/wp-json\/wp\/v2\/enfermedad?post=12809"},{"taxonomy":"framework","embeddable":true,"href":"https:\/\/www.iqac.csic.es\/en\/wp-json\/wp\/v2\/framework?post=12809"},{"taxonomy":"linea-de-investigacion","embeddable":true,"href":"https:\/\/www.iqac.csic.es\/en\/wp-json\/wp\/v2\/linea-de-investigacion?post=12809"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}