LIPIDOMICS
Scientific Supervisor: Josefina Casas
The Lipidomics Core Facility conducts comprehensive analysis of cellular lipids that encompass fatty acyls, glycerolipids, glycerophospholipids and sphingolipids. Such studies are possible with the advent of sophisticated mass spectrometric techniques. The services include sample preparation, lipid identification by mass spectrometry, LC-MS lipid profiling, LC-MS lipid quantification, and training in sample preparation methods.
Josefina Casas
Scientific Supervisor
josefina.casas@iqac.csic.es
Determination of the sphingolipid composition.
Addition of internal standards, extraction, purification and analysis by UPLC-TOF. Compounds are identified by its retention time and exact mass. The identification and quantification of sphingosine, sphinganine, sphingosine-1-phosphate and sphinganine-1-phosphate is carried out by HPLC-MS/ MS.
Class of compounds: Sphingosine and dihydrosphingosine, sphingosine-1-phosphate and sphinganine-1-phosphate, ceramide and dihydroceramide,sphingomyelin and dihydrosphingomyelin, hexosylceramide (glucosyl- and galactosyl-ceramide), lactosylceramide and dihydrolactosylceramide. Identification and relative amount of ganglioside molecular species.
Determination of the glycerolipid and glycerophospholipid composition.
Addition of internal standards, extraction, purification and analysis by UPLC-TOF. Compounds are identified by its retention time and exact mass. Available internal standards: triacylglicerol, diacylglycoreol, monoacylglicerol, phosphatidylcholine, plasmalogen, lysophosphatidylcholine, lysoplasmalogen, phosphatidylserine, lysophosphatidylserine,phosphatidylethanolamine, lysophosphatidylethanolamine. The number of carbon atoms and double bonds of the acyl groups will be indicated, but not it localization. It is possible to detect and quantify cholesteryl esters.
IQAC
Institut de Química Avançada de Catalunya
c/ Jordi Girona 18-26
08034 Barcelona - Spain
Ph: +34 93 400 61 00
Fax: +34 93 204 59 04