QUÍMICA D’ÀCIDS NUCLEICS
Cap de grup: Ramon Eritja
La síntesi d’àcids nucleics està en constant evolució i per això ens pot proporcionar un gran nombre d’eines molt versàtils amb diverses aplicacions tant en teràpia com en ciència dels materials. Els nostres estudis estan orientats a la millora del coneixement dels àcids nucleics mitjançant de la introducció de modificacions amb l’objectiu d’avaluar la seva eficàcia en teràpies tals com la teràpia antisentit i l’ARN d’interferència. Els projectes portats a terme pel nostre grup estan basats en estudis estructurals d’àcids nucleics amb especial èmfasi en aquells amb nucleòtids modificats o conjugats. A més a més, s’està treballant en nous agents de transfecció basats en els lípids catiònics per millorar l’administració dels àcids nucleics. Finalment, ressaltar el desenvolupament de noves molècules d’àcids nucleics per l’assemblatge de nanomaterials i la utilització de nous sistemes biosensors per detectar ADN i ARN biològicament rellevants.
Estructures no-canòniques i conjugats dels àcids nucleics
- Tríplex: modificacions químiques i estudis d’estabilitat estructural.
- Quàdruplex de guanina: modificacions químiques en els “loops” i les tètrades, noves arquitectures, disseny de lligands específics per seqüencies de rellevància biològica.
- “i-Motif”: caracterització fisico-química, disseny de lligands específics per seqüencies de rellevància biològica, avaluació quantitativa dels equilibris dúplex / “i-motif”
- Conjugats dels àcids nucleics: lípids, carbohidrats, agents intercalants, pèptids/PNA, colorants, polímers
- Oligonucleòtids que contenen bases non-naturals de rellevància estructural i biològica
Oligonucleòtids terapèutics
- RNA interferents, siRNA: millora de l’estabilitat, eficàcia de inhibició de la expressió gènica i selectivitat (reducció d’efectes no desitjats); caracterització estructural de les interaccions siRNA-proteïna; sistemes d’administració.
- ADN Antisense: modulació de la estabilitat a les nucleases; desenvolupament de sistemes d’administració utilitzant nous agents de transfecció.
- microRNA: miRNA mimètics i inhibidors (antagomirs)
- Aptàmers: increment del temps de vida mitja; transportadors de drogues citotòxiques.
Nanotecnologia de l’ADN (aplicacions biomèdiques i biosensors)
- Preparació d’estructures a escala nanomètrica dirigides por l’ADN (papiroflèxia de l’ADN o ADN “origami”, ADN tetraèdric).
- Nanomotlles d’ADN: Biosensors para la detecció de trombina i d’enzims de reparació de l’ADN; sistemes d’administració de drogues citotóxiques, motlles moleculars per al litografiat de superfícies d’or.
- Sondes basades en l’ADN para a la detecció de miRNA i de la metilació de l’ADN.
Personal científic
Ramon Eritja
Professor Investigació
ramon.eritja@iqac.csic.es
Carme Fàbrega
Científic Títular
carme.fabrega@iqac.csic.es
Personal de laboratori
Anna Mª Aviñó
Investigador CIBER
anna.avinyo@iqac.csic.es
Anna Clua Villas
Investigador Predoctoral
anna.clua@iqac.csic.es
Natalia Navarro
Investigador Predoctoral
natalia.navarro@iqac.csic.es
Arnau Domínguez
Investigador Predoctoral
arnau.dominguez@iqac.csic.es
Luis Miguel Menéndez
Investigador Predoctoral
luis.menendez@iqac.csic.es
Daniela Benigno
Investigadora Predoctoral
- Exploring the stabilizing effect on the i-motif of neighboring structural motifs and drugs-
Rodriguez, J., Domínguez, A., Aviñó, A., Borgonovo, G., Eritja, R., Mazzini, S., Gargallo, R.
International Journal of Biological Macromolecules, 242, art. no. 124794, 2023
- Evaluation of Poly(N-Ethyl Pyrrolidine Methacrylamide) (EPA) and Derivatives as Polymeric Vehicles for miRNA Delivery to Neural Cells-
Soto, A., Nieto-Díaz, M., Martínez-Campos, E., Noalles-Dols, A., Barreda-Manso, M.A., Reviriego, F., Reinecke, H., Reigada, D., Muñoz-Galdeano, T., Novillo, I., Gallardo, A., Rodríguez-Hernández, J., Eritja, R., Aviñó, A., Elvira, C., M. Maza, R.
Pharmaceutics, 15 (5), art. no. 1451, 2023
- Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclusters-
García, J.F., Reguera, D., Valls, A., Aviñó, A., Dominguez, A., Eritja, R., Gargallo, R.
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 297, art. no. 122752, 2023
- Lipid and Peptide-Oligonucleotide Conjugates for Therapeutic Purposes: From Simple Hybrids to Complex Multifunctional Assemblies-
Carme Fàbrega, Anna Aviñó, Natalia Navarro, Andreia F. Jorge, Santiago Grijalvo and Ramon Eritja
Pharmaceutics 2023, 15(2), 320
- Detection of SARS-CoV-2 Virus by Triplex Enhanced Nucleic Acid Detection Assay (TENADA)-
Anna Aviñó, Carlos Cuestas-Ayllón, Manuel Gutiérrez-Capitán, Lluisa Vilaplana, Valeria Grazu, Véronique Noé, Eva Balada, Antonio Baldi, Alex J. Félix, Eva Aubets, Simonas Valiuska, Arnau Domínguez, Raimundo Gargallo, Ramon Eritja, M.-Pilar Marco, César Fernández-Sánchez, Jesús Martínez de la Fuente and Carlos J. Ciudad
Int. J. Mol. Sci. 2022, 23(23), 15258
- Exploring the Interaction of G-quadruplex Binders with a (3 + 1) Hybrid G-quadruplex Forming Sequence within the PARP1 Gene Promoter Region-
Mazzini, S., Princiotto, S., Artali, R., Musso, L., Aviñó, A., Eritja, R., Gargallo, R., Dallavalle, S.
Molecules (Basel, Switzerland), 27 (15), 2022
- Properties of Parallel Tetramolecular G-Quadruplex Carrying N-Acetylgalactosamine as Potential Enhancer for Oligonucleotide Delivery to Hepatocytes-
Clua, A., Grijalvo, S., Erande, N., Gupta, S., Yucius, K., Gargallo, R., Mazzini, S., Manoharan, M., Eritja, R.
Molecules, 27 (12), art. no. 3944, 2022
- Oligonucleotides Carrying Nucleoside Antimetabolites as Potential Prodrugs-
Fàbrega, C., Clua, A., Eritja, R., Aviñó, A.
Current Medicinal Chemistry, 30 (11), pp. 1304-1319, 2023
- A multivalent Ara-C-prodrug nanoconjugate achieves selective ablation of leukemic cells in an acute myeloid leukemia mouse model-
Pallarès, V., Unzueta, U., Falgàs, A., Aviñó, A., Núñez, Y., García-León, A., Sánchez-García, L., Serna, N., Gallardo, A., Alba-Castellón, L., Álamo, P., Sierra, J., Cedó, L., Eritja, R., Villaverde, A., Vázquez, E., Casanova, I., Mangues, R.
Biomaterials, 280, art. no. 121258, 2022
- Chemical Modifications in Nucleic Acids for Therapeutic and Diagnostic Applications-
Fàbrega, C., Aviñó, A., Eritja, R.
Chemical Record, 2021
- Correlation between biophysical properties of niosomes elaborated with chloroquine and different tensioactives and their transfection efficiency-
Sainz-Ramos, M., Villate-Beitia, I., Gallego, I., Qtaish, N.A.L., Menéndez, M., Lagartera, L., Grijalvo, S., Eritja, R., Puras, G., Pedraz, J.L.
Pharmaceutics, 13 (11), art. no. 1787, 2021
- Biodistribution of 68/67Ga-radiolabeled sphingolipid nanoemulsions by pet and spect imaging-
Díez-Villares, S., Pellico, J., Gómez-Lado, N., Grijalvo, S., Alijas, S., Eritja, R., Herranz, F., Aguiar, P., de la Fuente, M.
International Journal of Nanomedicine, 16, pp. 5923-5935. 2021
- Investigation of the complexes formed between parp1 inhibitors and parp1 g-quadruplex at the gene promoter region-
Dallavalle, S., Princiotto, S., Mattio, L.M., Artali, R., Musso, L., Aviñó, A., Eritja, R., Pisano, C., Gargallo, R., Mazzini, S.
International Journal of Molecular Sciences, 22 (16), art. no. 8737, 2021
- Exploring the interaction of curaxin cbl0137 with g-quadruplex dna oligomers-
Dallavalle, S., Mattio, L.M., Artali, R., Musso, L., Aviñó, A., Fàbrega, C., Eritja, R., Gargallo, R., Mazzini, S.
International Journal of Molecular Sciences, 22 (12), art. no. 6476, 2021
- Rational engineering of a human GFP-like protein scaffold for humanized targeted nanomedicines-
Álamo, P., Cedano, J., Conchillo-Sole, O., Cano-Garrido, O., Alba-Castellon, L., Serna, N., Aviñó, A., Carrasco-Diaz, L.M., Sánchez-Chardi, A., Martinez-Torró, C., Gallardo, A., Cano, M., Eritja, R., Villaverde, A., Mangues, R., Vazquez, E., Unzueta, U.
Acta Biomaterialia, 130, pp. 211-222. 2021
- Evaluation of floxuridine oligonucleotide conjugates carrying potential enhancers of cellular uptake-
Aviñó, A., Clua, A., Bleda, M.J., Eritja, R., Fàbrega, C.
International Journal of Molecular Sciences, 22 (11), art. no. 5678, 2021
- Preparation of Lipid-Conjugated siRNA Oligonucleotides for Enhanced Gene Inhibition in Mammalian Cells-
Santiago Grijalvo, Sandra M. Ocampo, José Carlos Perales, Ramon Eritja
Ditzel H.J., Tuttolomondo M., Kauppinen S. (eds) Design and Delivery of SiRNA Therapeutics. Methods in Molecular Biology, vol 2282. Humana, New York, NY.
- EcoRII Restriction Endonuclease Forms Specific Contacts to the Bases of Its Target Sequence Flipped from DNA in a Transition Complex with Photoactivatable Substrates-
Kirsanova, O.V., Subach, F.V., Loiko, A.G., Eritja, R.I., Gromova, E.S.
Russian Journal of Bioorganic Chemistry, 47 (2), pp. 367-375. 2021
- Structural Effects of Incorporation of 2'‐Deoxy‐2'2'‐Difluorodeoxycytidine (Gemcitabine) in A‐ and B‐Form Duplexes-
Cristina Cabrero, Nerea Martín-Pintado, Stefania Mazzini, Raimundo Gargallo, Ramon Eritja, Anna Aviñó and Carlos González
Chem. Eur. J.. 27 (26), pp. 7351-7355. 2021
- The gene silencing of IRF5 and BLYSS effectively modulates the outcome of experimental lupus nephritis-
Jordi Guiteras, Élia Ripoll, Núria Bolaños, Laura De Ramon, Pere Fontova, Núria Lloberas, Josep Maria Cruzado, Josep Maria Aràn, Anna Aviñó, Ramon Eritja, Montse Gomà, Rosario Taco, Josep Maria Grinyó, Juan Torras
Molecular Therapy: Nucleic Acids Vol. 24 June 2021
- Parallel G-quadruplex Structures Increase Cellular Uptake and Cytotoxicity of 5-Fluoro-2'-deoxyuridine Oligomers in 5-Fluorouracil Resistant Cells-
Clua, A., Fàbrega, C., García-Chica, J., Grijalvo, S., Eritja, R.
Molecules (Basel, Switzerland), 26 (6), 2021
- Studies on the interactions of Ag(i) with DNA and their implication on the DNA-templated synthesis of silver nanoclusters and on the interaction with complementary DNA and RNA sequences-
Hoz, A.D.L., Navarro, A., Aviñó, A., Eritja, R., Gargallo, R.
RSC Advances, 11 (15), pp. 9029-9042. 2021
- G-quadruplex binding properties of a potent PARP-1 inhibitor derived from 7-azaindole-1-carboxamide-
Dallavalle, S., Musso, L., Artali, R., Aviñó, A., Scaglioni, L., Eritja, R., Gargallo, R., Mazzini, S.
Scientific Reports, 11 (1), art. no. 3869, 2021
- Oligonucleotides Containing 1-Aminomethyl or 1-Mercaptomethyl-2-deoxy- d -ribofuranoses: Synthesis, Purification, Characterization, and Conjugation with Fluorophores and Lipids-
Martín-Nieves, V., Fàbrega, C., Guasch, M., Fernández, S., Sanghvi, Y.S., Ferrero, M., Eritja, R.
Bioconjugate Chemistry, 2021
- An Androsterone‐H2@C60 hybrid: Synthesis, Properties and Molecular Docking Simulations with SARS‐Cov‐2-
Suárez, M., Makowski, K., Lemos, R., Almagro, L., Rodríguez, H., Herranz, M.Á., Molero, D., Ortiz, O., Maroto, E., Albericio, F., Murata, Y. and Martín, N.
ChemPlusChem. 2021
- Sorting hidden patterns in nanoparticle performance for glioblastoma using machine learning algorithms-
Basso, J., Mendes, M., Silva, J., Cova, T., Luque-Michel, E., Jorge, A.F., Grijalvo, S., Gonçalves, L., Eritja, R., Blanco-Prieto, M.J., Almeida, A.J., Pais, A., Vitorino, C.
International Journal of Pharmaceutics, 592, art. no. 120095, 2021
- Tuning g-quadruplex nanostructures with lipids. Towards designing hybrid scaffolds for oligonucleotide delivery-
Grijalvo, S., Clua, A., Eres, M., Gargallo, R., Eritja, R.
International Journal of Molecular Sciences, 22 (1), art. no. 121, pp. 1-24. 2021
- Synthesis of oligonucleotides carrying modified bases for DNA and protein recognition-
Fàbrega, C., Eritja, R.
Nucleic Acids Chemistry: Modifications and Conjugates for Biomedicine and Nanotechnology, pp. 87-141. 2021. Book Chapter
- Oligonucleotide conjugates and DNA nanotechnology-
Jorge, A.F., Grijalvo, S., Aviñó, A., Fàbrega, C., Eritja, R.
Nucleic Acids Chemistry: Modifications and Conjugates for Biomedicine and Nanotechnology, pp. 331-358. 2021. Book Chapter
- Nucleic acids chemistry: Modifications and conjugates for biomedicine and nanotechnology-
Eritja R
Nucleic Acids Chemistry: Modifications and Conjugates for Biomedicine and Nanotechnology, pp. 1-372. 2021. Book Chapter
- Advances in therapeutic oligonucleotide chemistry-
Jorge, A.F., Grijalvo, S., Aviñó, A., Fàbrega, C., Eritja, R.
Nucleic Acids Chemistry: Modifications and Conjugates for Biomedicine and Nanotechnology, pp. 273-329. 2021. Book Chapter
- Nucleic acids quadruplex-
Gargallo, R., González, C., Fàbrega, C., Aviñó, A., Eritja, R.
Nucleic Acids Chemistry: Modifications and Conjugates for Biomedicine and Nanotechnology, pp. 231-271. 2021. Book Chapter
- Nonradioactive labeling of oligonucleotides and postsynthetic modification of oligonucleotides-
Aviñó, A., Fàbrega, C., Eritja, R.
Nucleic Acids Chemistry: Modifications and Conjugates for Biomedicine and Nanotechnology, pp. 143-186. 2021. Book Chapter
- Nucleic acids triple helices-
Eritja R
Nucleic Acids Chemistry: Modifications and Conjugates for Biomedicine and Nanotechnology, pp. 187-230. 2021. Book Chapter
- Synthesis of oligonucleotides carrying DNA lesions for DNA repair studies-
Fàbrega, C., Eritja, R.
Nucleic Acids Chemistry: Modifications and Conjugates for Biomedicine and Nanotechnology, pp. 45-85, 2021, Book chapter
- Developing Protein-Antitumoral Drug Nanoconjugates as Bifunctional Antimicrobial Agents-
Serna, N., Carratalá, J.V., Parladé, E., Sánchez-Chardi, A., Aviñó, A., Unzueta, U., Mangues, R., Eritja, R., Ferrer-Miralles, N., Vazquez, E., Villaverde, A.
ACS Applied Materials and Interfaces, 12 (52), pp. 57746-57756. 2020
- Triplex hybridization-based nanosystem for the rapid screening of pneumocystis pneumonia in clinical samples-
Pla, L., Aviñó, A., Eritja, R., Ruiz-Gaitán, A., Pemán, J., Friaza, V., Calderón, E.J., Aznar, E., Martínez-Máñez, R., Santiago-Felipe, S.
Journal of Fungi, 6 (4), art. no. 292, pp. 1-14. 2020
- Design and engineering of tumor-targeted, dual-acting cytotoxic nanoparticles-
Voltà-Durán, E., Serna, N., Sánchez-García, L., Aviñó, A., Sánchez, J.M., López-Laguna, H., Cano-Garrido, O., Casanova, I., Mangues, R., Eritja, R., Vázquez, E., Villaverde, A., Unzueta, U.
Acta Biomaterialia, 119, pp. 312-322. 2020
- Study of alkaloid berberine and its interaction with the human telomeric i-motif DNA structure-
Gargallo, R., Aviñó, A., Eritja, R., Jarosova, P., Mazzini, S., Scaglioni, L., Taborsky, P.
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, art. no. 119185, . 2021 - Non-viral mediated gene therapy in human cystic fibrosis airway epithelial cells recovers chloride channel functionality-
Sainz-Ramos, M., Villate-Beitia, I., Gallego, I., A.L. Qtaish, N., Lopez-Mendez, T.B., Eritja, R., Grijalvo, S., Puras, G., Pedraz, J.L.
International Journal of Pharmaceutics, 588, art. no. 119757, 2020
- Influence of pH and a porphyrin ligand on the stability of a G-quadruplex structure within a duplex segment near the promoter region of the SMARCA4 gene-
Navarro, A., Benabou, S., Eritja, R., Gargallo, R.
International Journal of Biological Macromolecules, 159, pp. 383-393. 2020
- Dynamics-function analysis in catalytic RNA using NMR spin relaxation and conformationally restricted nucleotides-
Hoogstraten, C.G., Terrazas, M., Aviñó, A., White, N.A., Sumita, M.
Methods in Molecular Biology, 2167, pp. 183-202. 2020
- Detection of a g-quadruplex as a regulatory element in thymidylate synthase for gene silencing using polypurine reverse hoogsteen hairpins-
Aubets, E., Félix, A.J., Garavís, M., Reyes, L., Aviñó, A., Eritja, R., Ciudad, C.J., Noé, V.
International Journal of Molecular Sciences, 21 (14), art. no. 5028, pp. 1-22. 2020
- Fast and accurate pneumocystis pneumonia diagnosis in human samples using a label-free plasmonic biosensor-
Calvo-Lozano, O., Aviñó, A., Friaza, V., Medina-Escuela, A., Huertas, C.S., Calderón, E.J., Eritja, R., Lechuga, L.M.
Nanomaterials, 10 (6), art. no. 1246, pp. 1-18. 2020
- Brain Angiogenesis Induced by Nonviral Gene Therapy with Potential Therapeutic Benefits for Central Nervous System Diseases-
Gallego, I., Villate-Beitia, I., Soto-Sánchez, C., Menéndez, M., Grijalvo, S., Eritja, R., Martínez-Navarrete, G., Humphreys, L., López-Méndez, T., Puras, G., Fernández, E., Pedraz, J.L.
Mol. Pharmaceutics 2020, 17, 6, 1848–1858
- Sulfonamide as amide isostere for fine-tuning the gelation properties of physical gels-
Alegre-Requena, J.V., Grijalvo, S., Sampedro, D., Mayr, J., Saldías, C., Marrero-Tellado, J.J., Eritja, R., Herrera, R.P., Díaz, D.D.
RSC Advances, 10 (19), pp. 11481-11492. 2020
- Niosome-Based Approach for In Situ Gene Delivery to Retina and Brain Cortex as Immune-Privileged Tissues-
Al Qtaish, N., Gallego, I., Villate-Beitia, I., Sainz-Ramos, M., López-Méndez, T.B., Grijalvo, S., Eritja, R., Soto-Sánchez, C., Martínez-Navarrete, G., Fernández, E., Puras, G., Pedraz, J.L.
Pharmaceutics, 12 (3), art. no. 198, 2020
- Alginate Hydrogels as Scaffolds and Delivery Systems to Repair the Damaged Spinal Cord-
Grijalvo, S., Nieto-Díaz, M., Maza, R.M., Eritja, R., Díaz, D.D.
Biotechnology Journal, 14 (12), art. no. 1900275, 2019
- A pH-dependent bolt involving cytosine bases located in the lateral loops of antiparallel G-quadruplex structures within the SMARCA4 gene promotor-
Benabou, S., Mazzini, S., Aviñó, A., Eritja, R., Gargallo, R.
Scientific Reports, 9 (1), art. no. 15807, 2019
- Aptamer-peptide conjugates as a new strategy to modulate human α-thrombin binding affinity-
Aviñó, A., Jorge, A.F., Huertas, C.S., Cova, T.F.G.G., Pais, A., Lechuga, L.M., Eritja, R., Fabrega, C.
Biochimica et Biophysica Acta - General Subjects, 1863 (10), pp. 1619-1630. 2019
- Expanding the limits of amide-triazole isosteric substitution in bisamide-based physical gels-
Tautz, M., Torras, J., Grijalvo, S., Eritja, R., Saldías, C., Alemán, C., Díaz, D.D.
RSC Advances, 9 (36), pp. 20841-20851. 2019
- Study of conformational transitions of i-motif DNA using time-resolved fluorescence and multivariate analysis methods-
Benabou, S., Ruckebusch, C., Sliwa, M., Aviñó, A., Eritja, R., Gargallo, R., de Juan, A.
Nucleic acids research, 47 (13), pp. 6590-6605. 2019
- The Origins and the Biological Consequences of the Pur/Pyr DNA·RNA Asymmetry-
Terrazas, M., Genna, V., Portella, G., Villegas, N., Sánchez, D., Arnan, C., Pulido-Quetglas, C., Johnson, R., Guigó, R., Brun-Heath, I., Aviñó, A., Eritja, R., Orozco, M.
Chem, 5 (6), pp. 1619-1631. 2019
- Small interfering RNAs (siRNAs) in cancer therapy: a nano-based approach-
Mahmoodi Chalbatani, G., Dana, H., Gharagouzloo, E., Grijalvo, S., Eritja, R., Logsdon, C.D., Memari, F., Miri, S.R., Rad, M.R., Marmari, V.
International journal of nanomedicine, 14, pp. 3111-3128. 2019
- On the race for more stretchable and tough hydrogels-
Grijalvo, S., Eritja, R., Díaz, D.D.
Gels, 5 (2), art. no. 24, 24 p. 2019
- Efficient bioactive oligonucleotide-protein conjugation for cell-targeted cancer therapy-
Aviñó, A., Unzueta, U., Virtudes Céspedes, M., Casanova, I., Vázquez, E., Villaverde, A., Mangues, R., Eritja, R.
ChemistryOpen, 8 (3), pp. 382-387. 2019
- Parallel Clamps and Polypurine Hairpins (PPRH) for Gene Silencing and Triplex-Affinity Capture: Design, Synthesis, and Use-
Aviñó, A., Eritja, R., Ciudad, C.J., Noé, V.
Current Protocols in Nucleic Acid Chemistry, art. no. e78, .2019
- Cationic Niosomes as Non-Viral Vehicles for Nucleic Acids: Challenges and Opportunities in Gene Delivery-
Grijalvo, S., Puras, G., Zárate, J., Sainz-Ramos, M., Qtaish, N.A.L., López, T., Mashal, M., Attia, N., Díaz, D., Pons, R., Fernández, E., Pedraz, J.L., Eritja, R.
Pharmaceutics, 11 (2), art. no. 50,2019
- Overview of DNA Self-Assembling: Progresses in Biomedical Applications-
Jorge, A.F., Eritja, R.
Pharmaceutics, 10 (4), art. no. 268, .2018
- Selective depletion of metastatic stem cells as therapy for human colorectal cancer-
Céspedes, M.V., Unzueta, U., Aviñó, A., Gallardo, A., Álamo, P., Sala, R., Sánchez-Chardi, A., Casanova, I., Mangues, M.A., Lopez-Pousa, A., Eritja, R., Villaverde, A., Vázquez, E., Mangues, R.
EMBO Molecular Medicine, 10 (10), art. no. e8772, 2018
- Design of oligonucleotide-capped mesoporous silica nanoparticles for the detection of miRNA-145 by duplex and triplex formation-
Ribes, À., Santiago-Felipe, S., Aviñó, A., Candela-Noguera, V., Eritja, R., Sancenón, F.,Martínez-Máñez, R., Aznar, E.
Sensors and Actuators, B: Chemical, 277, pp. 598-603. 2018
- Naturally occurring quaternary benzo[: C] phenanthridine alkaloids selectively stabilize G-quadruplexes-
Jarosova, P., Paroulek, P., Rajecky, M., Rajecka, V., Taborska, E., Eritja, R., Aviñó, A., Mazzini, S., Gargallo, R., Taborsky, P.
Physical Chemistry Chemical Physics, 20 (33), pp. 21772-21782.2018
- The influence of the polar head-group of synthetic cationic lipids on the transfection efficiency mediated by niosomes in rat retina and brain-
Ojeda, E., Puras, G., Agirre, M., Zarate, J., Grijalvo, S., Eritja, R., Martínez-Navarrete, G., Soto-Sánchez, C., Diaz-Tahoces, A., Aviles-Trigueros, M., Fernández, E., Pedraz, J.L.
Biomaterials, 77, 267-279 (2016).
- Oligonucleotide delivery: a patent review (2010-2013)-
Grijalvo, S., Aviñó, A., Eritja, R.
Expt. Opin. Ther. Patents, 24(7), 801-819 (2014).
- Quadruplex nanostructures of d(TGGGGT): influence of sodium and potassium ions Pontinha, A.D., Chiorcea-Paquim, A.M., Eritja, R., Oliveira-Brett, A.M.-
Anal. Chem., 86(12), 5851-5857 (2014).
- Cationic vesicles based on non-ionic surfactant and synthetic aminolipids mediate delivery of antisense oligonucleotides into mammalian cells-
Grijalvo, S., Alagia, A., Puras, G., Zarate, J., Pedraz, J.L., Eritja, R.
Coll. Surf. B: Biointerfaces, 119, 30-37 (2014).
- A novel cationic niosome formulation for gene delivery to the retina-
Puras, G., Mashal, M., Zárate, J., Agirre, M., Ojeda, E., Grijalvo, S., Eritja, R., Díaz-Tahoces, A., Martínez Navarette, G., Avilés-Trigueros, M., Fernández, E., Pedraz, J.L.
Control. Rel., 174(1), 27-36 (2014).
- Solution equilibria of cytosine- and guanine-rich sequences near the promoter region of the n-myc gene that contain stable hairpins within lateral loops-
Benabou, S., Ferreira, R., Aviñó, A., González, C., Lyonnais, S., Solà, M., Eritja, R., Jaumot, J., Gargallo, R.
Biochim. Biophys. Acta (General subjects), 1840, 41-52 (2014).
- siRNA Modified with 2'-Deoxy-2'-C-methylpyrimidine Nucleosides-
Dellafiore, M., Aviñó, A., Alagia, A., Montserrat, J.M., Iribarren, A.M., Eritja, R.ChemBioChem, 19(13):1409-1413 (2018).
- Evaluation of the effect of polymorphism on G-quadruplex-ligand interaction by means of spectroscopic and chromatographic techniques-
Benito, S., Ferrer, A., Benabou, S., Aviñó, A., Eritja, R., Gargallo, R.Spectrochim Acta A Mol Biomol Spectrosc, 196, pp. 185-195 (2018).
- DNA-based nanoscaffolds as vehicles for 5-fluoro-2’-deoxyuridine oligomers in colorectal cancer therapy-
Jorge, A.F., Aviñó, A., Pais, A.A.C.C., Eritja, R., Fàbrega, C.
Nanoscale 2018, 10(15), 7238-7249.
- Glucose-nucleobase pairs within DNA: impact of hydrophobicity, alternative linking unit and DNA polymerase nucleotide insertion studies-
Vengut-Climent, E., Peñalver, P., Lucas, R., Gómez-Pinto, I., Aviñó, A., Muro-Pastor, A., Galbis, E., de Paz, V., Fonseca Guerra, C., Bickelhaupt, M., Eritja, R., González, C., Morales, J.C.
Chem. Sci. 2018, 9, 3544-3554 .
- Stem-cell based gene delivery mediated by cationic niosomes for bone regeneration purposes.-
Attia, N., Mashal, M., Grijalvo, S., Eritja, R., Zárate, J., Puras, G., Pedraz, J.L.
Nanomedicine: Nanotechnology, Biology, and Medicine 2018, 14(2), 521-531.
- Isosteric Substitution of 4 H-1,2,4-Triazole by 1 H-1,2,3-Triazole in Isophthalic Derivative Enabled Hydrogel Formation for Controlled Drug Delivery-
Häring, M., Rodríguez-López, J., Grijalvo, S., Tautz, M., Eritja, R., Martín, V.S., Díaz Díaz, D.
Molec Pharm (2018), 15 (8), 2963-2972.
- Covalent strategies for targeting messenger and non-coding RNAs: An updated review on siRNA, miRNA and antimiR conjugates-
Grijalvo, S., Alagia, A., Jorge, A.F., Eritja, R.Genes, 9 (2), art. no. 74, (2018).
- Exploring PAZ/3’-overhang interaction to improve siRNA specificity. A combined experimental and modeling study.-
Alagia, A., Jorge, A.F., Aviño, A., Cova, T.F.G.G., Crehuet, R., Grijalvo, S., Alberto Pais, A.A.C.C., Eritja, R.
Chem. Sci. 2018, 9, 2074-2086.
- Cationic nioplexes-in-polysaccharide-based hydrogels as versatile biodegradable hybrid materials to deliver nucleic acids.-
Grijalvo, S., Alagia, A., Puras, G., Zárate, J., Mayr, J., Pedraz, J. L., Eritja, R., Díaz Díaz, D.
Mat. Chem. B, 2017, 5, 7756-7767
- Boronic acid-modified alginate enables direct formation of injectable, self-healing and multistimuli-responsive hydrogel-
Pettignano, A., Grijalvo, S., Häring, M., Eritja, R., Tanchoux, N., Quignard, F., Díaz Díaz, D.
Chem. Commm. 2017, 53(23), 3350-3353.
- Oligonucleotide-lipid conjugates forming G-quadruplex structures are potent and pangenotypic hepatitis C virus entry inhibitors in vitro and ex vivo-
Koutsoudakis, G., Paris de León, A., Herrera, C., Dorner, M., Pérez-Vilaró, G., Lyonnais, S., Grijalvo, S., Eritja, R., Meyerhans, A., Mirambeau, G., Díez, J.
Antimicrob. Agents Chemother. 2017, 61(5), e02354-16.
- DNA-origami-driven lithography for patterning on gold surfaces with sub-10 nanometer resolution-
Gállego, I., Manning, B., Prades, J.D., Mir, M., Samitier, J., Eritja, R.
Adv. Mat. 2017, 29(11), 1603233.
- The effect of L-thymidine, acyclic thymine and 8-bromoguanine on the stability of model G-quadruplex structures-
Aviñó, A., Mazzini, S., Fàbrega, C., Peñalver, P; Gargallo, R.; Morales, J. C., Eritja, R.
Biochim. Biophys. Acta (General Subjects) 2017, 1861, 1205-1212.
- Controlling the reversible assembly of liposomes through a multi-stimuli responsive anchored DNA-
Hernández-Ainsa, S., Ricci, M., Hilton, L., Aviñó, A., Eritja, R., Keyser, U. F.
Nano Lett. 2016, 16(7), 4462-4466 .
- Glucose-nucleobase pseudo base pairs as a new biomolecular interaction in a DNA context-
Vengut-Climent, E., Gómez-Pinto, I., Lucas, R., Peñalver, P., Aviñó, A., Fonseca-Guerra, C, Bickelhaupt, F.M.., Eritja, R., González, C., Morales, J.C.
Angew. Chem. Int. Ed. Engl., 55(30), 8643-8647 (2016).
- siRNA and RNAi optimization-
Alagia, A., Eritja, R.
WIREs RNA, 7(3), 316-29 (2016).
- Label-free electrochemical DNA sensor using click-functionalized PEDOT electrodes.-
Application to Hepatitis C virus detection
Galán, T., Prieto, B., Alvira, M., Eritja, R., Götz, G., Bäuerle. P., Samitier, J.
Biosens. Bioelectron., 74(1), 751-756 (2015).
- Direct covalent attachment of DNA microarrays by rapid thiol-ene Click chemistry.-
Escorihuela, J., Bañuls, M.J., Grijalvo, S., Eritja, R., Puchades, R., Maquieira, A.
Bioconjugate Chem., 25(3), 618-627 (2014).
- Desarrollo de una terapia de primera clase para el tratamiento de la artrosis basada en oligonucleótidos inhibidores de microARN. (Development of a first-in-class therapy for the treament of osteoarthritis (OA) based on microRNA inhibitor oligonucleotides, OSTEOATX).”-
Coordinator: Beatriz Llamusí, Arthex Biotech S.L.
Grant: CDTI, Colaboración público-privada 2021, CPP2021-008792
Period: 2022-2025
Funding: 99.536.00€ (Total 987445.10€)
- OLIGOFASTX: Plataforma integral para el desarrollo sostenible de terapias basadas en oligonucleótidos-
Coordinator: Ana Isabel Jimenez (Sylentis)
Grant: Proyectos “MISIONES CIENCIA E INNOVACIÓN”, CDTI, Nº de proyecto: MIG-20211028. Dos contratos de investigación con las empresas Sylentis Grupo Pharmamar, Aptatargets S.L.
Period 2022-2025
Funding:
- Diagnosis and treatment of SARS-COV-2 by triplex formation-
Coordinator: Dr. Ramon Eritja Casadellà (IQAC-CSIC)
Grant: Fundación La Marató de TV3, código proyecto 202110-30.
Period: 2021-2023
Funding: 140.000.00 €
- Estructuras de ADN y sistemas de administración de medicamentos.-
Coordinator: Ramon Eritja Casadella
Grant: PID2020-118145RB-I00 funded by MCIN/ AEI/10.13039/501100011033
Period: 2021-2024
Funding: 164.000,00
