Research | Technological Platforms

Lipidomics

Lipidomics is the large-scale study of lipids, which are essential molecules in any biological system. This analysis provides key information on cell signaling, metabolic pathways, and the development of various diseases.

We offer a comprehensive lipidomics service focused on the precise characterization, identification, and quantification of lipids in biological samples. We are a specialized team with extensive experience in the analysis of mammalian samples, and we also have expertise in other systems such as yeast, viruses, or plants.

To ensure maximum sensitivity and specificity, we use high-resolution mass spectrometry techniques via a UPLC system coupled to a Cyclic IMS with cyclic ion mobility. This advanced technology allows us to separate and identify lipids with high resolution, improving their subsequent quantification.

The primary objective of the service is to provide quality data that helps interpret the complexity of lipid pathways and their functional impact. The service is designed to meet the needs of both academic researchers and biotechnology companies.

Contact: selip@iqac.csic.es

Lipid analysis

We perform a comprehensive analysis of multiple lipid subclasses, including:

  • Fatty acyls (fatty acids and endocannabinoids)
  • Glycerolipids
  • Phospholipids
  • Sphingolipids
  • Sterols

For each subclass, the different lipid species are determined based on the associated fatty acids and the number of unsaturations.

Workflow

We offer a complete service that includes the preparation, extraction, analysis, and quantification of the different lipids present in the sample. Additionally, we provide personalized scientific advice at all stages of the project, from experimental design to the interpretation of results.

  • Reception and pre-preparation
    Depending on the project’s needs, we offer protein quantification to normalize the results.
  • Lipid extraction
    We apply specific protocols adapted to each type of sample to ensure optimal recovery of the lipids to be analyzed.
  • LC-MS analysis
    We use mass spectrometry through two systems: a UPLC-cIMS-QTOF unit, which allows for high-resolution lipid separation and identification, and a UPLC-TQ-MS system, suitable for targeted analysis and quantification of specific lipids.
  • Processing and quantification
    We identify and quantify lipid species in a targeted manner, using internal and external standards.
  • Delivery of results
    We provide a detailed report in Excel format with the list of identified and quantified lipid species, as well as summary graphs that facilitate biological interpretation.

Staff

Alejandro Iñáñez Blas

Technical staff

Alexandre Garcia Barrera

Technical staff

Mireia Casasampere Ferrer

Technical staff