PROTEOMICS AND IMAGING
Scientific Supervisor: Carme Quero
The IQAC-Proteomics Service is focused on the analysis of biomolecules (proteins, peptides, protein modifications, oligonucleotides, sugars,…) and large organic molecules (such as polymers, dendrimers, polyphenols and other macromolecules) by MALDI-TOF/TOF mass spectrometry.
Most recently, the Service also offers MALDI Imaging. This technique allows direct measurement of these biomolecules as well as drugs and metabolites distribution in tissues. The great advantage of MALDI Imaging is that the distribution of the detected compounds can be visualized as images, which can be integrated with other imaging modalities. This method is label-free and allows multiplex analysis of several molecules in the same tissue section simultaneously.
The Service offers additional services related to the analysis of other molecules and development of methods and protocols.
Carme Quero
Scientific Supervisor
carme.quero@iqac.csic.es
Identification of proteins by Peptide Mass Fingerprinting (PMF) and peptide fragmentation by MALDI-TOF/TOF MS.
- In-gel tryptic digestions of 1D Bands and 2D Spots or proteins in solution.
- Analysis of the peptides by MALDI-TOF MS, getting the peptide maps.
- Fragmentation of peptides (1 to 3) (MS / MS)
Separation of proteins by one-dimensional electrophoresis (SDS-PAGE) and separation of complex protein samples using the technique of two-dimensional electrophoresis (2D-PAGE).
Use of electrophoretic techniques for separating proteins. For the separation according to their molecular weight
This technique allows the separation of proteins based on two features: its isoelectric point (Isoelectric focusing, IEF) and molecular weight (electrophoresis under denaturing conditions, SDS-PAGE).
MALDI Imaging
Label-free measurement of biomolecules, drugs and metabolites distribution in tissues. Integration with other imaging modalities. Multiplex analysis of several molecules in the same tissue section simultaneously.
Molecular mass determination by MALDI-TOF mass spectrometry
Analysis of biomolecules (proteins, peptides, oligonucleotides, sugars …) and large organic molecules (such as polymers, dendrimers, polyphenols and other macromolecules) by MALDI-TOF/TOF mass spectrometry.
IQAC
Institut de Química Avançada de Catalunya
c/ Jordi Girona 18-26
08034 Barcelona - Spain
Ph: +34 93 400 61 00
Fax: +34 93 204 59 04